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After blocking in 5% skimmed milk powder for 2 hours at room temperature, the samples were incubated overnight at 4C with primary antibodies, including ERK1/2 (#9102), p-ERK1/2 (#9101), JNK1/2 (#9252), p-JNK (#9251), p38 MAPK (#9212), and p-p38 MAPK (#9211), which were obtained from Cell Signaling Technology (Beverly, MA, USA), as well as VEGF-a (sc-7269) and Tubulin (sc-8035), which were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA)
Thats why its pushed at the end of treatment
Deepmala, et al
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ii) the capacity to analyze a greater number of samples in a more efficient manner, with a reaction time of merely 30 min, and compatibility with 96-well plate formats for the rapid and simultaneous analysis of a high volume of samples and iii) the absence of any expertise requirement, due to the simplicity of the test
